Protein Purification
INNOVATIVE BIOCHIPS Single Cell Isolation Chip: 1CellDish-60mm, 64 wells, Pack of 5
1CellDish is a handheld device with microfluidics aliquoting channels designed for single-cell isolation. The operation is by pipetting cell suspension into the center well and distributing cells to the satellite wells to form single-cell aliquots in less than 2 µL volumes. Features: 1) It provides rapid, convenient, and damage-free cell isolation. 2) It simplifies steps for harvesting target cells and genes. 3) It is compatible with all cell sizes smaller than 50 µm at either low or high cell density. 4) It uniformly distributes cell suspension into 64 outlet wells and retrieves single target cells by pipetting. Parameters: 1) Size of device: standard 60 mm Petri Dish format. 2) Number of wells: 64. 3) Well volume: 2.5 µL. 4) Single-cell yield: about 20 cells per device. Summary: 1CellDish isolates cells at a price below $5 per cell. It is used for single-cell lysis, PCR, sequencing, and multiomics.
ABclonal Technology Acetyl-Histone H3-K9
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes This structure consists of approximately 146 bp of DNA wrapped around a nucleosome an octamer composed of pairs of each of the four core histones (H2A H2B H3 and H4) The chromatin fiber is further compacted through the interaction of a linker histone H1 with the DNA between the nucleosomes to form higher order chromatin structures This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family Transcripts from this gene lack polyA tails instead they contain a palindromic termination element This gene is found in the large histone gene cluster on chromosome 6p22-p21 3
Cell Signaling Technology SimpleChIP Universal qPCR Master Mix 1 ml
SimpleChIP Universal qPCR Master Mix 1 ml
Enzo Life Sciences Aflatoxicol (1mg). CAS: 29611-03-8
Rare Aflatoxin that is similar but less toxic than Aflatoxin B1. Exposure may produce necrosis, cirrhosis and carcinoma of the liver. Induces DNA damage. In vitro metabolite of Aflatoxin B1. Alternative name: Aflatoxin R0. Purity: ≥95% (HPLC). Solubility: Soluble in DMSO, ethyl acetate, methanol, ethanol, or petrol ether. Not soluble in water. Long Term Storage: -20°C.
Hampton Research CRYSTAL SCREEN 2 NO13 185ML
HR2-912-13/Crystal Screen 2 #13 (F01), 185 ml - 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol monomethyl ether 2,000. Please note items from this supplier are non-returnable.
Hampton Research CRYSTAL SCREEN 2 NO02 185ML
HR2-912-02/Crystal Screen 2 #02 (E02), 185 ml - 0.5 M Sodium chloride, 0.01 M Magnesium chloride hexahydrate, 0.01 M Hexadecyltrimethylammonium bromide. Please note items from this supplier are non-returnable.
Zymo Research Corporation ChIP DNA Clean & Concentrator, ZR-96, 2 x 96 Preps
The ZR-96 Chromatin Immunoprecipitation (ChIP) DNA Clean & Concentrator offers a rapid, high-throughput method for purifying and concentrating high-quality DNA from standard ChIP protocols, including reverse cross-linked and enzyme-digested samples. It also purifies DNA from PCR and other enzymatic reactions, making it suitable for PCR, Next-Gen sequencing (ChIP-Seq), and other molecular applications.
- Rapid high-throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K-digested samples, PCR, and other enzymatic reactions.
- Plate design permits DNA elution at high concentrations into minimal elution volumes (> 10 μL/well).
- Eluted DNA is well-suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.
Invent Biotechnologies Inc Minute™ Cytosolic and Nuclear Extraction Kit for Frozen/Fresh Tissues (20 Preps)
Preparation of cytosolic and nuclear fractions from cultured cells and tissues is common. Cell fractionation from cultured cells is relatively easy and straightforward. However, a clear separation of cytosolic and nuclear fractions from tissues, especially frozen tissues, is challenging. The cross-contamination from frozen tissues is always an issue because of the altered tissue cellular structure caused by the freeze and thaw cycle, improper homogenization, and the use of an inadequate extraction buffer. More information: https://inventbiotech.com/products/minute-cytosolic-and-nuclear-extraction-kit-for-frozen-fresh-tissues?_pos=1&_sid=2257e16f3&_ss=r
Invent Biotechnologies Inc Minute Single Nucleus Isolation Kit for Tissues/Cells, Catalog Number SN-047, Processing Time ~30 min, Minimum Tissue Input 1 mg, Recommended Tissue Input 15-20 mg, Minimum Cell Input 200,000 cells, Recommended Cell Input 4-5 million cells, 20 preps
The Minute Single Nucleus Isolation Kit is designed for the efficient isolation and purification of single nuclei from various tissue and cell types. This kit overcomes common challenges in traditional nuclei isolation, such as aggregation and doublet formation, by providing a simple, rapid, and highly effective protocol. It is ideal for applications that require high-quality, well-separated single nuclei, including FACS analysis, single nucleus RNA-seq, immunofluorescence staining, and cell cycle analysis.
- Enhanced single nuclei yield to minimize aggregation and doublet formation.
- Rapid processing time of approximately 30 minutes for nuclei isolation.
- Broad compatibility with fresh or frozen tissues and cultured cells.
- Low input requirement that works with 1-30 mg of tissue.
- Suitable for diverse downstream applications such as RNA-seq and ATAC-seq.
Hampton Research HR2-906-24/StockOptions Bis-Tris #24 pH 7.8, 185 ml
HR2-906-24/StockOptions Bis-Tris #24 pH 7.8, 185 ml. Please note items from this supplier are non-returnable.
Zymo Research Corporation EndoZero™ Spin Columns (5 Pack)
EndoZero Spin-Columns is the fastest and simplest method available to efficiently isolate endotoxin-free plasmid DNA from E. coli.
Zymo Research Corporation Zymo Spin™ II-P (50 Pack)
Features polypropylene construction and contains a unique silica-based matrix that allows purification of up to 100 g of plasmid DNA in 25 l eluate when used in combination with ZymoPURE™ Plasmid buffers. Capacity is 800 l.
Zymo Research Corporation Zymo-Spin™ IIICG Columns (250 Pack) (Capped) Green Retension Ring
The Zymo-Spin IIICG features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 g of DNA, and 100 g of RNA, in 50l eluate. Capacity is 800 l.